Testing the Use of Salmonid Primers for Polymerase Chain Reaction in Various Species of Fish

Testing the Use of Salmonid Primers for Polymerase Chain Reaction in Various Species of Fish

Mitochondrial DNA is a useful tool for measuring the genetic distance between different species or the genetic diversity within a single species. In order to obtain this information from the DNA, it must first be amplified by polymerase chain reaction, which requires specific primers to copy the DNA. This experiment tested how effectively Salmonid specific primers could be used to amplify the mitochondrial DNA of various species of fish including Chinook salmon, pink salmon, hybrid pinook salmon, Atlantic salmon, brook trout, lake herring, chub, walleye, Lake Whitefish, and yellow perch. It was found that while the primers being tested amplified the DNA of the Chinook, pink, hybrid, and Atlantic salmon, it did not amplify the DNA of the other species.

Alexander, Jennifer

Biology

Lake Superior State University

2013

Dr. Nancy Kirkpatrick

Copyright Jennifer Alexander: All rights reserved. LSSU use only.

application/pdf

English

text.monograph

S20230110001