Bactericidal Activity of Salmonid Serum

Bactericidal Activity of Salmonid Serum

"The bacterial killing ability of the complement system in the serum of Atlantic salmon (Salmo salar), pink salmon (Onchorynchus gorbuscha), and lake trout (Salvelinus namaycush) was examined. The purpose was to determine whether there was a difference between the bactericidal activity of serum obtained from these three different fish species. The sera were diluted using phosphate buffered saline with calcium and magnesium ions (PBS/Ca2+Mg2+). Serial dilutions of a model bacteria (Escherichia coli) were incubated with the diluted sera and then inoculated onto agar plates. After two to three days, the colonies on these plates were counted and the survival rates of the E. coli in the different incubation conditions were calculated. A difference was observed between the survival rate of the E. coli incubated with the fish sera and the negative control (incubated with PBS/Ca2+Mg2+), as well as between the E. coli incubated with fish serum and the positive control (fetal bovine serum). However, there was no significant difference for the bactericidal activity of the serum from the three fish species."

Alexander, Ashley

Biology

Lake Superior State University

2015

Dr. Jun Li

Copyright Ashley Alexander: All rights reserved. LSSU use only.

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S20220517010